Service Description Purified genomic DNA is fragmented using a Covaris R230 AFA instrument. Whole genome libraries are prepared using IDT XGen DNAseq reagents on a Beckman Coulter Biomek i7 liquid handling platform. Finished libraries are pooled (typically 8-plex) and exome hybridization capture enrichment is performed using Twist Biosciences probes and reagents. Sequencing is performed with paired-end 150bp reads to specified depth of coverage on an Illumina NovaSeq X Plus.
Sample Requirements:
DNA Quality:
DNA Amount: 200ng (it is possible to generate libraries from much less DNA, however this will result in reduced library complexity (ie fewer unique library molecules after PCR) and as a result after removing PCR duplicates during analysis, desired X coverage may not be possible.
Volume: >10ul
Buffer: EB (10mM Tris-Cl, pH 8.5) or MolBio. grade nuclease free water. Buffers containing EDTA should be avoided because it may inhibit enzymatic reactions.