The Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer Institute (DFCI) offers a full suite of services for a wide variety of genomic next-generation sequencing (NGS) library preparation, sequencing and analysis applications including RNAseq, ChIPseq, ATACseq, CRISPR screening, whole genome methylation profiling, targeted resequencing, single-cell RNAseq, exome sequencing, and more. The genomics core technology platforms include Illumina NextSeq500s, MiSeqs, a MiniSeq, NovaSeq 6000, and NovaSeq X Plus. High throughput sample preparation is performed on Beckman Coulter Biomek FX and i7 systems. Low throughput precious samples are prepared by experienced technical staff. The MBCF writes and maintains standardized pipelines for routine NGS analysis such as RNAseq, ChIPseq, and CRISPR screening. Complex bioinformatics analysis such as integration of multi-omics datasets or specialized analyses can be performed by the experienced computational biology team.
Note on Data Transfer and Storage
Historically low cost of generating NGS data has enabled applications requiring deeper sequencing and projects with larger numbers of samples. Although the prices have come down, the volume and size of the data files has increased significantly. As a result, data transfer, storage, and analysis are non-trivial factors of an NGS project that should be given serious consideration. Please visit our Data Transfer page for more details.
In the meantime, here are important questions to answer even before submitting samples: