MBCF Services
The Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer Institute offers a full suite of services for a wide variety of genomic next-generation sequencing (NGS) library preparation, sequencing and analysis applications including RNAseq, ChIPseq, ATACseq, CRISPR screening, whole genome methylation profiling, targeted resequencing, single-cell RNAseq, exome sequencing, and more. The genomics core technology platforms include Illumina MiSeqs and NovaSeq X Plus. High throughput sample preparation is performed on Beckman Coulter Biomek i7 systems. Low throughput precious samples are prepared by experienced technical staff. The MBCF writes and maintains standardized pipelines for routine NGS analysis such as RNAseq, ChIPseq, ATACseq, variant calling, and CRISPR screening. Complex bioinformatics analysis such as integration of multi-omics datasets or specialized analyses can be performed by the experienced computational biology team.
Note on Data Transfer and Storage
Historically low cost of generating NGS data has enabled applications requiring deeper sequencing and projects with larger numbers of samples. Although the prices have come down, the volume and size of the data files has increased significantly. As a result, data transfer, storage, and analysis are non-trivial factors of an NGS project that should be given serious consideration. Please visit our Data Transfer page for more details.
In the meantime, here are important questions to answer even before submitting samples:
- Where are you planning to store your data?
- Where are you planning to analyze your data?
- What is your preferred transfer method?
Service Menu
Note: MBCF does not support library preparation -- Sequencing Only
- 10X gene expression (GEX)
- 10X scATACseq
- 10X Multiome GEX
- 10X Multiome ATAC