Methylation Profiling

Whole Genome Methylation Profiling

Please note these services are only performed by request, and requirements, cost, and deliverables will vary based on experimental goals. Please contact us at mbcfgenomics@mail.harvard.edu to schedule a free consultation. 

Enzymatic Methylation Sequencing (EMSeq)

Enzymatic Methylation Sequencing (EMSeq) is a method used to detect and quantify DNA methylation across the genome using enzymatic conversion rather than bisulfite treatment. This approach enables sensitive and accurate analysis of methylation patterns at base-pair resolution. Now automated!

Other Flavors:

Reduced Representation Enzymatic Methylation Sequencing (RR-EMSeq)

Reduced Representation Enzymatic Methylation Sequencing (RR-EMSeq) enriches for CpG-rich regions of the genome, allowing for cost-effective and focused DNA methylation profiling via enzymatic conversion. This method provides high-resolution methylation analysis in targeted genomic regions.

Target Capture EMSeq

Target Capture EMSeq combines enzymatic methylation sequencing with hybridization-based target enrichment to focus on specific genomic regions of interest. This enables detailed methylation analysis within selected regions, enhancing sensitivity and reducing sequencing costs. This service is only performed by request; please contact us for a quote​

Bisulfite Conversion

Bisulfite conversion is a chemical treatment that deaminates unmethylated cytosines to uracil, allowing for the detection of DNA methylation when followed by sequencing. This approach is widely used for genome-wide and targeted methylation studies at single-base resolution.​