Low Input TotalRNAseq ​(rRNA depletion)​

Service Description

Sample Requirements:

• RNA Quality: DV200 > 40%
• DNase Treated purified RNA
• MBCF optimal input : 250pg-10ng (>1ng recommended)
• Volume: >10ul 

Analytical Goals:

• Gene expression profiling, differential expression testing between conditions (replicates required!), pathway, gene ontology and gene set enrichment analysis
• suitable for degraded RNA (e.g FFPE) and non-polyA non-coding RNAs

Other Information:

• Strand specific
• Receive 50M 150bp read pairs per sample
• User Manual: SMARTer Stranded Total RNAseq Kit v3 - Pico Input Mammalian

Recommended Bioinformatics Pipeline: Viper (Visualization Pipeline for RNAseq)