ChIPseq / Cut & Run

Service Description

Chromatin immunoprecipitation sequencing (ChIPseq) is a method used to analyze DNA-protein interactions. Typical targets are histone protein modifications and transcription factors (TFs).

Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is a technique used to profile DNA-binding proteins and chromatin modifications with high resolution. This method employs a targeted micrococcal nuclease (MNase) to selectively cleave DNA at protein-binding sites, enabling precise mapping of protein-DNA interactions.

Sample Requirements:

• MBCF optimal input : >1ng DNA is ideal, but our sample preparation method is sensitive enough to produce informative data from less.
• Volume & amount : >10ul 

MBCF QC Protocol: 

• DNA is often limited and therefore we do not perform QC on the initial sample, but instead QC rely on the initial library yield and size distribution to determine whether the sample is likely to produce informative data — for this application they almost always do!
• In order to preserve samples, we use 1/2 of the volume submitted. The remaining enriched DNA can be returned upon request for validation experiments. Alternatively, in the rare event of library prep failure for technical reasons, the remaining volume may also be used to re-prep the libraries (with your consent).

Other Information:

• ChIPseq- Receive 40M 150bp read pairs per sample
• Cut & Run- Receive 20M 150bp read pairs per sample

Recommended Bioinformatics Pipeline: Peak Calling Pipeline